Rhusonoside A, a New Megastigmane Glycoside from Rhus sylvestris, Increases the Function of Osteoblastic MC3T3-E1 Cells

Abstract

Chemical investigation of the stems and leaves of Rhus sylvestris afforded a new megastigmane glycoside named rhusonoside A (1), along with four other known compounds: dihydroquercetin (2), astragalin (3), hyperin (4), and kaempferol-3-O-rutinoside (5). Their structures were determined by a combination of spectroscopic analysis and application of the modified Mosher’s method. The effect of compounds 1 – 5 on the function of osteoblastic MC3T3-E1 cells was examined by determining cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Rhusonoside A (1) significantly increased the function of osteoblastic MC3T3-E1 cells. Cell viability, ALP activity, and collagen synthesis were increased dose dependently, up to 155.39 %, 171.27 %, and 134.25 %, respectively, of the basal value at 10 μM (P < 0.05). In addition, 0.1 μM of compound 1 significantly increased mineralization of MC3T3-E1 cells to 142.78 % (P < 0.05) of the basal value.

Key words

Rhus sylvestris - Anacardiaceae - megastigmane glycoside - modified Mosher’s method - osteoporosis - osteoblastic MC3T3-E1 cells

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